Whole transcriptome profiling of prospective endomyocardial biopsies reveals prognostic and diagnostic signatures of cardiac allograft rejection.

ABSTRACT:

Background

Heart transplantation provides a significant improvement in survival and quality of life for patients with end-stage heart disease, however many recipients experience different levels of graft rejection that can be associated with significant morbidities and mortality. Current clinical standard-of-care for the evaluation of heart transplant acute rejection (AR) consists of routine endomyocardial biopsy (EMB) followed by visual assessment by histopathology for immune infiltration and cardiomyocyte damage. We assessed whether the sensitivity and/or specificity of this process could be improved upon by adding RNA sequencing (RNA-seq) of EMBs coupled with histopathological interpretation.

Methods

Up to 6 standard-of-care, or for-cause EMBs, were collected from 26 hear

SUBMITTER: Piening BD 

PROVIDER: S-EPMC9133065 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature