Determining protein polarization proteome-wide using physical dissection of individual Stentor coeruleus cells.

ABSTRACT: Cellular components are non-randomly arranged with respect to the shape and polarity of the whole cell.^1-4 Patterning within cells can extend down to the level of individual proteins and mRNA.^5,6 But how much of the proteome is actually localized with respect to cell polarity axes? Proteomics combined with cellular fractionation^7-11 has shown that most proteins localize to one or more organelles but does not tell us how many proteins have a polarized localization with respect to the large-scale polarity axes of the intact cell. Genome-wide localization studies in yeast^12-15 found that only a few percent of proteins have a localized position relative to the cell polarity axis defined by sites of polarized cell growth. Here, w

SUBMITTER: Lin A 

PROVIDER: S-EPMC9133221 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature