Blockade of TGF-β (Transforming Growth Factor Beta) Signaling by Deletion of Tgfbr2 in Smooth Muscle Cells of 11-Month-Old Mice Alters Aortic Structure and Causes Vasomotor Dysfunction-Brief Report.

ABSTRACT:

Background

To test the hypothesis that smooth muscle cell (SMC) TGF-β (transforming growth factor beta) signaling contributes to maintenance of aortic structure and function beyond the early postnatal period.

Methods

We deleted the TBR2 (type 2 TGF-β receptor) in SMC of 11-month-old mice (genotype Acta2-CreER^T2+/0Tgfbr2^f/f, termed TBR2^SMΔ) and compared their ascending aorta structure and vasomotor function to controls (Acta2-CreER^T20/0Tgfbr2^f/f, termed TBR2^f/f).

Results

We confirmed loss of aortic SMC TBR2 by immunoblotting. Four weeks after SMC TBR2 loss, TBR2^SMΔ mice did not have aortic rupture, ulceration, dissection, dilation, or evidence of medial hemorrhage.

SUBMITTER: Lee CY 

PROVIDER: S-EPMC9133230 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature