Rational engineering of industrial S. cerevisiae: towards xylitol production from sugarcane straw.

ABSTRACT:

Background

Sugarcane hemicellulosic material is a compelling source of usually neglected xylose that could figure as feedstock to produce chemical building blocks of high economic value, such as xylitol. In this context, Saccharomyces cerevisiae strains typically used in the Brazilian bioethanol industry are a robust chassis for genetic engineering, given their robustness towards harsh operational conditions and outstanding fermentation performance. Nevertheless, there are no reports on the use of these strains for xylitol production using sugarcane hydrolysate.

Results

Potential single-guided RNA off-targets were analyzed in two preeminent industrial strains (PE-2 and SA-1), providing a database of 5'-NGG 20 nucleotide sequences and guidelines for the fast and cost-effectiv

SUBMITTER: de Mello FDSB 

PROVIDER: S-EPMC9133290 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature