DNAzyme Sensor Uses Chemiluminescence Resonance Energy Transfer for Rapid, Portable, and Ratiometric Detection of Metal Ions.

ABSTRACT: DNAzymes have emerged as an important class of sensors for a wide variety of metal ions, with florescence DNAzyme sensors as the most widely used in different sensing and imaging applications because of their fast response time, high signal intensity, and high sensitivity. However, the requirements of an external excitation light source and its associated power increase the cost and size of the fluorometer, making it difficult to be used for portable detections. To overcome these limitations, we report herein a DNAzyme sensor that relies on chemiluminescence resonance energy transfer (CRET) without the need for external light. The sensor is constructed by combining the functional motifs from both Pb²⁺-dependent 8-17 DNAzyme conjugated to fluorescein (FAM) and hemin/G-quadruplex

SUBMITTER: Zheng J 

PROVIDER: S-EPMC9133356 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature