Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice.

ABSTRACT: Oocytes and granulosa cells closely interact with each other during follicular development, and a lack of appropriate signaling between them results in infertility. Attempts to manipulate oocyte microenvironment have been impeded by the impermeability of the blood-follicle barrier (BFB). To establish a strategy for manipulating oogenesis, we use adeno-associated viruses (AAVs), which have a unique ability of transcytosis. Microinjecting of AAVs into the ovarian stroma penetrates the BFB and achieves long-term gene expression. Introduction of an AAV carrying the mouse Kitl gene restores oogenesis in congenitally infertile Kitl^Sl-t/Kitl^Sl-t mutant mouse ovaries, which lack Kitl expression but contain only primordial follicles. Healthy offspring without AAV integration a

SUBMITTER: Kanatsu-Shinohara M 

PROVIDER: S-EPMC9133397 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature