Transcriptional profiling of macrophages in situ in metastatic melanoma reveals localization-dependent phenotypes and function.

ABSTRACT: Modulation of immune function at the tumor site could improve patient outcomes. Here, we analyze patient samples of metastatic melanoma, a tumor responsive to T cell-based therapies, and find that tumor-infiltrating T cells are primarily juxtaposed to CD14⁺ monocytes/macrophages rather than melanoma cells. Using immunofluorescence-guided laser capture microdissection, we analyze transcriptomes of CD3⁺ T cells, CD14 ⁺ monocytes/macrophages, and melanoma cells in non-dissociated tissue. Stromal CD14⁺ cells display a specific transcriptional signature distinct from CD14⁺ cells within tumor nests. This signature contains LY75, a gene linked with antigen capture and regulation of tolerance and immunity in dendritic cells (DCs). When applied

SUBMITTER: Martinek J 

PROVIDER: S-EPMC9133468 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature