Presynaptic Mitochondria Communicate With Release Sites for Spatio-Temporal Regulation of Exocytosis at the Motor Nerve Terminal.

ABSTRACT: Presynaptic Ca²⁺ regulation is critical for accurate neurotransmitter release, vesicle reloading of release sites, and plastic changes in response to electrical activity. One of the main players in the regulation of cytosolic Ca²⁺ in nerve terminals is mitochondria, which control the size and spread of the Ca²⁺ wave during sustained electrical activity. However, the role of mitochondria in Ca²⁺ signaling during high-frequency short bursts of action potentials (APs) is not well known. Here, we studied spatial and temporal relationships between mitochondrial Ca²⁺ (mCa²⁺) and exocytosis by live imaging and electrophysiology in adult motor nerve terminals of transgenic mice expressing synaptophysin-pHluorin (SypHy). Our results

SUBMITTER: Lopez-Manzaneda M 

PROVIDER: S-EPMC9133601 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature