Autophagy Regulation Influences β-Amyloid Toxicity in Transgenic Caenorhabditis elegans.

ABSTRACT: Alzheimer's disease (AD) is a progressive, neurodegenerative disease characterized by the accumulation of amyloid-beta (Aβ) proteins in the form of plaques that cause a proteostasis imbalance in the brain. Several studies have identified autophagy deficits in both AD patients and AD animal models. Here, we used transgenic Caenorhabditis elegans to study the relationship between autophagy flux and Aβ. We labeled autophagosomes with an advanced fluorescence reporter system, and used this to observe that human Aβ expression caused autophagosome accumulation in C. elegans muscle. The autophagy-related drugs chloroquine and 3-MA were employed to investigate the relationship between changes in autophagic flux and the toxicity of Aβ expression. We found that reducing autophagosome a

SUBMITTER: Lin H 

PROVIDER: S-EPMC9133694 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature