Protposer: The web server that readily proposes protein stabilizing mutations with high PPV.

ABSTRACT: Protein stability is a requisite for most biotechnological and medical applications of proteins. As natural proteins tend to suffer from a low conformational stability ex vivo, great efforts have been devoted toward increasing their stability through rational design and engineering of appropriate mutations. Unfortunately, even the best currently used predictors fail to compute the stability of protein variants with sufficient accuracy and their usefulness as tools to guide the rational stabilisation of proteins is limited. We present here Protposer , a protein stabilising tool based on a different approach. Instead of quantifying changes in stability, Protposer uses structure- and sequence-based screening modules to nominate candidate mutations for subseq

SUBMITTER: Garcia-Cebollada H 

PROVIDER: S-EPMC9133766 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature