Characterization and Sequence Mapping of Large RNA and mRNA Therapeutics Using Mass Spectrometry.

ABSTRACT: Large RNA including mRNA (mRNA) has emerged as an important new class of therapeutics. Recently, this has been demonstrated by two highly efficacious vaccines based on mRNA sequences encoding for a modified version of the SARS-CoV-2 spike protein. There is currently significant demand for the development of new and improved analytical methods for the characterization of large RNA including mRNA therapeutics. In this study, we have developed an automated, high-throughput workflow for the rapid characterization and direct sequence mapping of large RNA and mRNA therapeutics. Partial RNase digestions using RNase T1 immobilized on magnetic particles were performed in conjunction with high-resolution liquid chromatography-mass spectrometry analysis. Sequence mapping was performed using automated

SUBMITTER: Vanhinsbergh CJ 

PROVIDER: S-EPMC9134182 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature