De Novo Design of the ArsR Regulated P_ars Promoter Enables a Highly Sensitive Whole-Cell Biosensor for Arsenic Contamination.

ABSTRACT: Whole-cell biosensors for arsenic contamination are typically designed based on natural bacterial sensing systems, which are often limited by their poor performance for precisely tuning the genetic response to environmental stimuli. Promoter design remains one of the most important approaches to address such issues. Here, we use the arsenic-responsive ArsR-P_ars regulation system from Escherichia coli MG1655 as the sensing element and coupled gfp or lacZ as the reporter gene to construct the genetic circuit for characterizing the refactored promoters. We first analyzed the ArsR binding site and a library of RNA polymerase binding sites to mine potential promoter sequences. A set of tightly regulated P_ars promoters by ArsR was designed

SUBMITTER: Chen SY 

PROVIDER: S-EPMC9134189 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature