A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence of Chlamydomonas telomeric DNA.

ABSTRACT: Competition among DNA non-canonical structures has been widely studied in repetitive DNA sequences. The factors affecting DNA structural polymorphism have always been an important area of research. The Chlamydomonas reinhardtii telomere (TTTTAGGG) _n is found to be an exception to the general idea of forming a folded G-quadruplex by a few repeats in any telomeric sequence. Herein, using gel electrophoresis, UV thermal melting, UV thermal difference spectra (TDS), circular dichroism, and fluorescence and NMR spectroscopy. We demonstrated that the double repeat of the C. reinhardtii telomere (TTTTAGGG)₂ (Chlm2) adopts an intramolecular non-conventional triplex structure in Na⁺ solution. We report that the co-existence of reverse Hoogsteen

SUBMITTER: Bansal A 

PROVIDER: S-EPMC9134377 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature