Harnessing changes in open chromatin determined by ATAC-seq to generate insulin-responsive reporter constructs.

ABSTRACT:

Background

Gene regulation is critical for proper cellular function. Next-generation sequencing technology has revealed the presence of regulatory networks that regulate gene expression and essential cellular functions. Studies investigating the epigenome have begun to uncover the complex mechanisms regulating transcription. Assay for transposase-accessible chromatin by sequencing (ATAC-seq) is quickly becoming the assay of choice for many epigenomic investigations. However, whether intervention-mediated changes in accessible chromatin determined by ATAC-seq can be harnessed to generate intervention-inducible reporter constructs has not been systematically assayed.

Results

We used the insulin signaling pathway as a model to investigate chromatin regions and gene expression c

SUBMITTER: Merrill CB 

PROVIDER: S-EPMC9134605 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature