Neurotensin differentially regulates bile acid metabolism and intestinal FXR-bile acid transporter axis in response to nutrient abundance.

ABSTRACT: Studies demonstrate a role for neurotensin (NT) in obesity and related comorbidities. Bile acid (BA) homeostasis alterations are associated with obesity. We determined the effect of NT on BA metabolism in obese and non-obese conditions. Plasma and fecal BA profiles were analyzed by LC-MS/MS in male and female NT^+/+ and NT^-/- mice fed low-fat (LFD) or high-fat diet (HFD) for 6 weeks (early stage of obesity) or greater than 20 weeks (late stage of obesity). The nuclear farnesoid X receptor (FXR) and BA transporter mRNA expression were assessed in ileum, mouse enteroids, and human cell lines. HFD decreased plasma primary and secondary BAs in NT^+/+ mice; HFD-induced decrease of plasma BAs was improved in NT-deficient mice. In NT^+/+ mice, HFD inhibite

SUBMITTER: Li J 

PROVIDER: S-EPMC9134645 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature