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A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion.


ABSTRACT:

Background

Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapidly and simultaneously consume three major lignocellulosic sugars (i.e., glucose, xylose, and arabinose) faster than any previously reported microorganisms.

Results

The xylose isomerase pathway was constructed in Vibrio sp. dhg, which naturally displays high metabolic activities on glucose and arabinose but lacks xylose catabolism. Subsequent adaptive laboratory evolution significantly improved xylose catabolism of initial strain and led to unprecedently high growth and sugar uptake rate (0.67 h-1 and 2.15 g gdry cell weight-1 h-1, respectively). Furthermore, we achieved co-consumption of the three sugars by deletion of PtsG and introduction of GalP. We validated its superior performance and applicability by demonstrating efficient lactate production with high productivity (1.15 g/L/h) and titer (83 g/L).

Conclusions

In this study, we developed a Vibrio-based microbial platform with rapid and simultaneous utilization of the three major sugars from lignocellulosic biomass by applying an integrated approach of rational and evolutionary engineering. We believe that the developed strain can be broadly utilized to accelerate the production of diverse biochemicals from lignocellulosic biomass.

SUBMITTER: Woo S 

PROVIDER: S-EPMC9134653 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

A Vibrio-based microbial platform for accelerated lignocellulosic sugar conversion.

Woo Sunghwa S   Lim Hyun Gyu HG   Han Yong Hee YH   Park Sungwoo S   Noh Myung Hyun MH   Baek Dongyeop D   Moon Jo Hyun JH   Seo Sang Woo SW   Jung Gyoo Yeol GY  

Biotechnology for biofuels and bioproducts 20220525 1


<h4>Background</h4>Owing to increasing concerns about climate change and the depletion of fossil fuels, the development of efficient microbial processes for biochemical production from lignocellulosic biomass has been a key issue. Because process efficiency is greatly affected by the inherent metabolic activities of host microorganisms, it is essential to utilize a microorganism that can rapidly convert biomass-derived sugars. Here, we report a novel Vibrio-based microbial platform that can rapi  ...[more]

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