Quantifying Tissue-Specific Proteostatic Decline in Caenorhabditis elegans.

ABSTRACT: The ability to maintain proper function and folding of the proteome (protein homeostasis) declines during normal aging, facilitating the onset of a growing number of age-associated diseases. For instance, proteins with polyglutamine expansions are prone to aggregation, as exemplified with the huntingtin protein and concomitant onset of Huntington's disease. The age-associated deterioration of the proteome has been widely studied through the use of transgenic Caenorhabditis elegans expressing polyQ repeats fused to a yellow fluorescent protein (YFP). This polyQ::YFP transgenic animal model facilitates the direct quantification of the age-associated decline of the proteome through imaging the progressive formation of fluorescent foci (i.e., protein aggregates) and subsequent onset of locomot

SUBMITTER: Lazaro-Pena MI 

PROVIDER: S-EPMC9134844 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature