In vitro and in vivo anti-lymphoma effects of Ophiorrhiza pumila extract.

ABSTRACT:

Background

Current therapeutic strategies on patients with lymphomas remains limited. Previously we found the suppressive effect of Ophiorrhiza pumila (OPE) on hepatocarcinoma. In present study, the effect of OPE on lymphoma in vitro and in vivo were investigated.

Methods

CCK-8 assay was applied to detect the effect of OPE on cell proliferation. Flow cytometry was used to analyze the effect of OPE on cell cycle distribution and apoptosis. Xenograft mouse model was conducted to determine the anti-tumor activity of OPE. TNUEL assay was performed to detect the apoptosis in tumor tissues. Western blot and immuno-histochemistry were used to determine protein expression.

Results

In vitro tests indicate that OPE suppressed A20 cell proliferation i

SUBMITTER: Fan L 

PROVIDER: S-EPMC9134945 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature