Global profiling of protein lysine malonylation in Toxoplasma gondii strains of different virulence and genetic backgrounds.

ABSTRACT: Lysine malonylation is a post-translational modification (PTM), which regulates many cellular processes. Limited information is available about the level of lysine malonylation variations between Toxoplasma gondii strains of distinct genetic lineages. Yet, insights into such variations are needed to understand the extent to which lysine malonylation contributes to the differences in the virulence and repertoire of virulence factors between T. gondii genotypes. In this study, we profiled lysine malonylation in T. gondii using quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) and immuno-affinity purification. This analysis was performed on three T. gondii strains with distinctive pathogenicity in mice, including RH strain (type I), PRU strain (type II), and VEG strain (t

SUBMITTER: Nie LB 

PROVIDER: S-EPMC9135328 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature