Spatial transcriptomics reveals metabolic changes underly age-dependent declines in digit regeneration.

ABSTRACT: De novo limb regeneration after amputation is restricted in mammals to the distal digit tip. Central to this regenerative process is the blastema, a heterogeneous population of lineage-restricted, dedifferentiated cells that ultimately orchestrates regeneration of the amputated bone and surrounding soft tissue. To investigate skeletal regeneration, we made use of spatial transcriptomics to characterize the transcriptional profile specifically within the blastema. Using this technique, we generated a gene signature with high specificity for the blastema in both our spatial data, as well as other previously published single-cell RNA-sequencing transcriptomic studies. To elucidate potential mechanisms distinguishing regenerative from non-regenerative healing, we applied spatial transcriptomic

SUBMITTER: Tower RJ 

PROVIDER: S-EPMC9135401 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature