An adapted consensus protein design strategy for identifying globally optimal biotherapeutics.

ABSTRACT: Biotherapeutic optimization, whether to improve general properties or to engineer specific attributes, is a time-consuming process with uncertain outcomes. Conversely, Consensus Protein Design has been shown to be a viable approach to enhance protein stability while retaining function. In adapting this method for a more limited number of protein sequences, we studied 21 consensus single-point variants from eight publicly available CD3 binding sequences with high similarity but diverse biophysical and pharmacological properties. All single-point consensus variants retained CD3 binding and performed similarly in cell-based functional assays. Using Ridge regression analysis, we identified the variants and sequence positions with overall beneficial effects on developability attributes of the C

SUBMITTER: Liu Y 

PROVIDER: S-EPMC9135432 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature