A topological refactoring design strategy yields highly stable granulopoietic proteins.

ABSTRACT: Protein therapeutics frequently face major challenges, including complicated production, instability, poor solubility, and aggregation. De novo protein design can readily address these challenges. Here, we demonstrate the utility of a topological refactoring strategy to design novel granulopoietic proteins starting from the granulocyte-colony stimulating factor (G-CSF) structure. We change a protein fold by rearranging the sequence and optimising it towards the new fold. Testing four designs, we obtain two that possess nanomolar activity, the most active of which is highly thermostable and protease-resistant, and matches its designed structure to atomic accuracy. While the designs possess starkly different sequence and structure from the native G-CSF, they show specific activity in differe

SUBMITTER: Skokowa J 

PROVIDER: S-EPMC9135769 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature