Active Expression of Human Hyaluronidase PH20 and Characterization of Its Hydrolysis Pattern.

ABSTRACT: Hyaluronidases are a group of glycosidases catalyzing the degradation of hyaluronic acid (HA). Because of the advantages of effectively hydrolyzing the HA-rich matrix and low immunogenicity, human hyaluronidase PH20 (hPH20) is widely used in the medical field. Here, we realized the active expression of recombinant hPH20 by Pichia pastoris under a methanol-induced promoter P_AOX1. By optimizing the composition of the C-terminal domain and fusing protein tags, we constructed a fusion mutant AP₂-△491C with the extracellular hyaluronidase activity of 258.1 U·L^-1 in a 3-L bioreactor, the highest expression level of recombinant hPH20 produced by microbes. Furthermore, we found recombinant hPH20 hydrolyzed the β-1,4 glycosidic bonds sequentially from the red

SUBMITTER: Pang B 

PROVIDER: S-EPMC9136067 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature