Enhancing Long-Read-Based Strain-Aware Metagenome Assembly.

ABSTRACT: Microbial communities are usually highly diverse and often involve multiple strains from the participating species due to the rapid evolution of microorganisms. In such a complex microecosystem, different strains may show different biological functions. While reconstruction of individual genomes at the strain level is vital for accurately deciphering the composition of microbial communities, the problem has largely remained unresolved so far. Next-generation sequencing has been routinely used in metagenome assembly but there have been struggles to generate strain-specific genome sequences due to the short-read length. This explains why long-read sequencing technologies have recently provided unprecedented opportunities to carry out haplotype- or strain-resolved genome assembly. Here, we pr

SUBMITTER: Luo X 

PROVIDER: S-EPMC9136235 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature