Incorporation of reporter genes into mitochondrial DNA in budding yeast.
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ABSTRACT:
SUBMITTER: Rzepka M
PROVIDER: S-EPMC9136344 | biostudies-literature | 2022 Jun
REPOSITORIES: biostudies-literature
Publications
Incorporation of reporter genes into mitochondrial DNA in budding yeast.
Rzepka Magdalena M Suhm Tamara T Ott Martin M
STAR protocols 20220525 2
Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, <i>Saccharomyces cerevisiae</i>. Mitochondrially encoded reporter constructs can be used to interrogate various aspects of mitochondrial gene expression. The power of this technique is exemplified by a mitochondrially encoded nanoluciferase, which allows to monitor levels ...[more]
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