Transcriptional states and chromatin accessibility during bovine myoblasts proliferation and myogenic differentiation.

ABSTRACT:

Objectives

Although major advances have been made in bovine epigenome study, the epigenetic basis for fetal skeletal muscle development still remains poorly understood. The aim is to recapitulated the time course of fetal skeletal muscle development in vitro, and explore the dynamic changes of chromatin accessibility and gene expression during bovine myoblasts proliferation and differentiation.

Methods

PDGFR- cells were isolated from bovine fetal skeletal muscle, then cultured and induced myogenic differentiation in vitro in a time-course study (P, D0, D2,and D4). The assay for transposase-accessible chromatin sequencing (ATAC-seq) and RNA sequencing (RNA-seq) were performed.

Results

Among the enriched transcriptional factors with high variability, we determined the e

SUBMITTER: Li Q 

PROVIDER: S-EPMC9136495 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature