Long noncoding RNAs regulated spermatogenesis in varicocele-induced spermatogenic dysfunction.

ABSTRACT:

Objectives

To evaluate the expression, potential functions and mechanisms of long noncoding RNAs (lncRNAs) in the pathogenesis of varicocele (VC)-induced spermatogenic dysfunction.

Materials and methods

We established a rat model with left experimental VC and divided rats into the sham group, the VC group, and the surgical treatment group (each group, n = 10). Haematoxylin and eosin (HE) staining and sperm quality were analysed to evaluate spermatogenesis function. LncRNA expression profiles were analysed using lncRNA-Seq (each group n = 3) and validated using quantitative real-time polymerase chain reaction (each group n = 10). Correlation analysis and gene target miRNA prediction were used to construct competing endogenous RNA network. The regulated signalling pathway and

SUBMITTER: Wang S 

PROVIDER: S-EPMC9136499 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature