Dnmt1 is required for the development of auditory organs via cell cycle arrest and Fgf signalling.

ABSTRACT:

Objectives

To explore the role of DNA methyltransferase 1 (DNMT1) in the development of auditory system using zebrafish as experimental model.

Methods

Morpholino oligonucleotide was used to induce Dnmt1 deficiency. RNA sequencing, in situ hybridization (ISH), whole genomic bisulfide sequencing (WGBS) and immunostaining were used to investigate the morphologic alterations and mechanisms.

Results

We found that downregulation of Dnmt1 induced decreased number of neuromasts and repressed cell proliferation of primordium in the developing posterior lateral line system of zebrafish. The ISH data uncovered that Fgf signalling pathway was inhibited and the expression of chemokine members cxcr4b, cxcr7b and cxcl12a were interfered, while lef1 expression was increased after inh

SUBMITTER: Tang D 

PROVIDER: S-EPMC9136517 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature