Substrate Partitioning into Protein Macromolecular Frameworks for Enhanced Catalytic Turnover.

ABSTRACT: Spatial partitioning of chemical processes is an important attribute of many biological systems, the effect of which is reflected in the high efficiency of enzymes found within otherwise chaotic cellular environments. Barriers, often provided through the formation of compartments or phase segregation, gate the access of macromolecules and small molecules within the cell and provide an added level of metabolic control. Taking inspiration from nature, we have designed virus-like particles (VLPs) as nanoreactor compartments that sequester enzyme catalysts and have used these as building blocks to construct 3D protein macromolecular framework (PMF) materials, which are structurally characterized using small-angle X-ray scattering (SAXS). The highly charged PMFs form a separate phase in suspens

SUBMITTER: Selivanovitch E 

PROVIDER: S-EPMC9136710 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature