Assay establishment and validation of a high-throughput organoid-based drug screening platform.

ABSTRACT:

Background

Organoids are three-dimensional structures that closely recapitulate tissue architecture and cellular composition, thereby holding great promise for organoid-based drug screening. Although growing in three-dimensional provides the possibility for organoids to recapitulate main features of corresponding tissues, it makes it incommodious for imaging organoids in two-dimensional and identifying surviving organoids from surrounding dead cells after organoids being treated by irradiation or chemotherapy. Therefore, significant work remains to establish high-quality controls to standardize organoid analyses and make organoid models more reproducible.

Methods

In this study, the Z-stack imaging technique was used for the imaging of three-dimensional organoids to gather al

SUBMITTER: Li X 

PROVIDER: S-EPMC9137096 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature