Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome.

ABSTRACT: The human oral cavity is one of the hotspots harboring multiple mobile genetic elements (MGEs), which are segments of DNA that can move either within bacterial genomes or between bacterial cells that can facilitate the spreading of genetic materials, including antimicrobial resistance genes. It is, therefore, important to investigate genes associated with the MGEs as they have a high probability of dissemination within the bacterial population under selective pressure from human activities. As one-third of oral bacteria are not yet culturable in the laboratory condition, therefore, in this work, it is aimed to detect and identify the genetic contexts of MGEs in the oral cavity through an inverse PCR (IPCR)-based approach on the oral metagenomic. The human oral metagenome was extracted from

SUBMITTER: Tansirichaiya S 

PROVIDER: S-EPMC9137128 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature