Conserved RNA secondary structure in Cherry virus A 5'-UTR associated with translation regulation.

ABSTRACT:

Background

A variety of cis-acting RNA elements with structures in the 5'- or 3'-untranslated region (UTR) of viral genomes play key roles in viral translation. Cherry virus A (CVA) is a member of the genus Capillovirus in the family Betaflexiviridae. It has a positive single-stranded RNA genome of ~ 7400 nucleotides (nt). The length of the CVA 5'-UTR is ~ 100 nt; however, the function of this long UTR has not yet been reported.

Methods

Molecular and phylogenetic analyses were performed on 75 CVA sequences, which could be divided into four groups, and the RNA secondary structure was predicted in four CVA 5'-UTR types. These four CVA 5'-UTR types were then inserted upstream of the firefly luciferase reporter gene FLuc (FLuc), and in vitro translation of the corresponding tran

SUBMITTER: Wang D 

PROVIDER: S-EPMC9137147 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature