The granulosa cell response to luteinizing hormone is partly mediated by YAP1-dependent induction of amphiregulin.

ABSTRACT:

Background

The LH surge is a pivotal event that triggers multiple key ovarian processes including oocyte maturation, cumulus expansion, follicular wall rupture and luteinization of mural granulosa and theca cells. Recently, LH-dependent activation of the Hippo signaling pathway has been shown to be required for the differentiation of granulosa cells into luteal cells. Still, the precise interactions between Hippo and LH signaling in murine granulosa cells remain to be elucidated.

Methods

To detect the expression of effectors of the Hippo pathway, western blot, immunohistochemical and RT-qPCR analyses were performed on granulosa cells treated with LH in vitro or isolated from immature mice treated with eCG and hCG. Cultured granulosa cells were pretreated with pharmacologic i

SUBMITTER: Godin P 

PROVIDER: S-EPMC9137176 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature