Utility of ultra-rapid real-time PCR for detection and prevalence of Rickettsia spp. in ticks.

ABSTRACT:

Background

Rickettsia spp. are important tick-borne pathogens that cause various human and animal diseases worldwide. A tool for rapid and accurate detection of the pathogens from its vectors is necessary for prevention of Rickettsioses propagation in humans and animals, which are infested by ticks. Therefore, this study was conducted to evaluate a molecular tool, ultra-rapid real-time PCR (UR-qPCR), for rapid and accurate detection of Rickettsia spp. from 5644 ticks in 408 pools collected from livestock and their surrounding environments in Gangwon and Jeju province in South Korea.

Results

The UR-qPCR of Rickettsia DNA showed a limit of detection of 2.72 × 10¹ copies of Rickettsia DNA and no cross reaction with other tick-borne pathogens, namely Anaplasma phagocy

SUBMITTER: Truong AT 

PROVIDER: S-EPMC9137179 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature