Metabolomic Expression of Laryngeal and Hindlimb Muscles in Adult versus Senescent Rats.

ABSTRACT:

Objectives

(1) Determine the feasibility of obtaining a global, unbiased metabolomic profile on laryngeal muscle in a rat model; (2) evaluate the impact of biological aging on the laryngeal metabolome; and (3) characterize biochemical expression differences between aged and non-aged laryngeal and hindlimb muscle.

Methods

Thyroarytenoid laryngeal muscle and plantaris hindlimb muscle were harvested from 5 young adult (9 months old) and 5 older adult (32 months old) F344BN rats. Tissue was processed and analyzed using LC-MS methods. Detected metabolites were compared to widely used metabolite databases and KEGG pathway enrichment was performed on significant metabolites.

Results

The greatest differences in metabolite expression were between laryngeal and limb muscle with

SUBMITTER: Shembel AC 

PROVIDER: S-EPMC9137437 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature