Clostridioides difficile Toxin B PCR Cycle Threshold as a Predictor of Toxin Testing in Stool Specimens from Hospitalized Adults.

ABSTRACT: Rapid, accurate detection of Clostridioides difficile toxin may potentially be predicted by toxin B PCR cycle threshold (tcdB C_t). We investigated the validity of this approach in an inpatient adult population. Patients who tested positive by C. difficile PCR (Cepheid GeneXpert) from December 2016 to October 2020 (n = 368) at a tertiary medical center were included. All stool samples were further tested by rapid glutamate dehydrogenase (GDH)/toxin B EIA and cell cytotoxin neutralization assay (CCNA). Receiver operating characteristic curves were analyzed. The area under the curve for tcdB C_t predicting toxin result by EIA was 0.795 (95% confidence interval (CI) 0.747-0.843) and by CCNA was 0.771 (95% CI 0.720-0.822). The Youden C

SUBMITTER: Lee S 

PROVIDER: S-EPMC9137712 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature