Thioredoxin Reductase-Type Ferredoxin: NADP⁺ Oxidoreductase of Rhodopseudomonas palustris: Potentiometric Characteristics and Reactions with Nonphysiological Oxidants.

ABSTRACT: Rhodopseudomonas palustris ferredoxin:NADP⁺ oxidoreductase (RpFNR) belongs to a novel group of thioredoxin reductase-type FNRs with partly characterized redox properties. Based on the reactions of RpFNR with the 3-acetylpyridine adenine dinucleotide phosphate redox couple, we estimated the two-electron reduction midpoint potential of the FAD cofactor to be -0.285 V. 5-Deaza-FMN-sensitized photoreduction revealed -0.017 V separation of the redox potentials between the first and second electron transfer events. We examined the mechanism of oxidation of RpFNR by several different groups of nonphysiological electron acceptors. The k_cat/K_m values of quinones and aromatic N-oxides toward RpFNR increase with

SUBMITTER: Lesanavicius M 

PROVIDER: S-EPMC9137726 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature