Self-Assembled Hydrogel Microparticle-Based Tooth-Germ Organoids.

ABSTRACT: Here, we describe the characterization of tooth-germ organoids, three-dimensional (3D) constructs cultured in vitro with the potential to develop into living teeth. To date, the methods used to successfully create tooth organoids capable of forming functional teeth have been quite limited. Recently, hydrogel microparticles (HMP) have demonstrated utility in tissue repair and regeneration based on their useful characteristics, including their scaffolding ability, effective cell and drug delivery, their ability to mimic the natural tissue extracellular matrix, and their injectability. These outstanding properties led us to investigate the utility of using HMPs (average diameter: 158 ± 32 µm) derived from methacrylated gelatin (GelMA) (degree of substitution: 100%) to create tooth organoids.

SUBMITTER: Kilic Bektas C 

PROVIDER: S-EPMC9137977 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature