Dataset Information

MatriGrid^® Based Biological Morphologies: Tools for 3D Cell Culturing.

ABSTRACT: Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-called template or morphology transfer techniques. Over the last 15 years, in one such research venture, we have been investigating a micro thermoforming technique as a facile tool for generating bioinspired topographies. We call them MatriGrid^®s. In this research account, we summarize our learning outcome from this technique in terms of the influence of 3D micro morphologies on different cell cultures that we have tested in our laboratory. An integral part of this research is the evolution of unavoidable aspects such as possible label-free sensing and fluidic automatization. The development in the research field is also documented in this account.

SUBMITTER: Mai P

PROVIDER: S-EPMC9138054 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature

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Publications

MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing.

Mai Patrick P Hampl Jörg J Baca Martin M Brauer Dana D Singh Sukhdeep S Weise Frank F Borowiec Justyna J Schmidt André A Küstner Johanna Merle JM Klett Maren M Gebinoga Michael M Schroeder Insa S IS Markert Udo R UR Glahn Felix F Schumann Berit B Eckstein Diana D Schober Andreas A

Bioengineering (Basel, Switzerland) 20220520 5

Recent trends in 3D cell culturing has placed organotypic tissue models at another level. Now, not only is the microenvironment at the cynosure of this research, but rather, microscopic geometrical parameters are also decisive for mimicking a tissue model. Over the years, technologies such as micromachining, 3D printing, and hydrogels are making the foundation of this field. However, mimicking the topography of a particular tissue-relevant substrate can be achieved relatively simply with so-call ...[more]

PMID: 35621498

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Project description:BackgroundTo sustain high universal Long-Lasting Insecticidal Nets (LLINs) coverage, affordable nets that provide equivalent or better protection than standard LLINs, are required. Test facilities evaluating new LLINs require compliance to Good Laboratory Practice (GLP) standards to ensure the quality and integrity of test data. Following GLP principles allows for the reconstruction of activities during the conduct of a study and minimizes duplication of efficacy testing. This case study evaluated the efficacy of two LLINs: SafeNet NF® and SafeNet® LLIN.MethodsThe study was conducted according to GLP principles and followed World Health Organization guidelines for evaluating LLINs. The LLINs were assessed in experimental huts against wild, pyrethroid-resistant Anopheles arabiensis mosquitoes. Nets were either unwashed or washed 20 times and artificially holed to simulate a used torn net. Blood-feeding inhibition and mortality were compared with a positive control (Interceptor® LLIN) and an untreated net.ResultsMosquito entry in the huts was reduced compared to negative control for the unwashed SafeNet NF, washed Safenet LLIN and the positive control arms. Similar exiting rates were found for all the treatment arms. Significant blood-feeding inhibition was only found for the positive control, both when washed and unwashed. All insecticide treatments induced significantly higher mortality compared to an untreated net. Compared to the positive control, the washed and unwashed SafeNet NF® resulted in similar mortality. For the SafeNet® LLINs the unwashed net had an equivalent performance, but the mortality for the washed net was significantly lower than the positive control. Internal audits of the study confirmed that all critical phases complied with Standard Operating Procedures (SOPs) and the study plan. The external audit confirmed that the study complied with GLP standards.ConclusionsSafeNet NF® and SafeNet® LLIN offered equivalent protection to the positive control (Interceptor® LLIN). However, further research is needed to investigate the durability, acceptability, and residual efficacy of these nets in the community. This study demonstrated that GLP-compliant evaluation of LLINs can be successfully conducted by African research institutions.

Dataset Information

MatriGrid^® Based Biological Morphologies: Tools for 3D Cell Culturing.

Publications

MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing.

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Dataset Information

MatriGrid® Based Biological Morphologies: Tools for 3D Cell Culturing.

Publications

MatriGrid<sup>®</sup> Based Biological Morphologies: Tools for 3D Cell Culturing.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

MatriGrid^® Based Biological Morphologies: Tools for 3D Cell Culturing.