Multi-Color Two-Photon Microscopic Imaging Based on a Single-Wavelength Excitation.

ABSTRACT: Two-photon probes with broad absorption spectra are beneficial for multi-color two-photon microscopy imaging, which is one of the most powerful tools to study the dynamic processes of living cells. To achieve multi-color two-photon imaging, multiple lasers and detectors are usually required for excitation and signal collection, respectively. However, one makes the imaging system more complicated and costly. Here, we demonstrate a multi-color two-photon imaging method with a single-wavelength excitation by using a signal separation strategy. The method can effectively solve the problem of spectral crosstalk by selecting a suitable filter combination and applying image subtraction. The experimental results show that the two-color and three-color two-photon imaging are achieved with a single

SUBMITTER: Yan W 

PROVIDER: S-EPMC9138471 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature