High Expression of a tRNA^Pro Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence.

ABSTRACT: Colorectal cancer (CRC) is the second most lethal cause of cancer-related deaths in Europe. Fragments of tRNA^Pro are conserved among vertebrates, characterized by pleiotropic regulatory functions and have been found to discriminate colorectal tumors from normal colorectal mucosa. In the current study, we investigated the prognostic utility of 5'-tiRNA-Pro^TGG levels in CRC. For this purpose, total RNA was extracted from 155 malignant colorectal tumors and 74 adjacent non-cancerous tissue specimens, polyadenylated and reverse-transcribed using an oligo-dT adapter as primer. Real-time quantitative PCR (qPCR) was used to assess the levels of 5'-tiRNA-Pro^TGG. Kaplan-Meier survival analysis demonstrated that high 5'-tiRNA-Pro^TGG levels predict both poo

SUBMITTER: Tsiakanikas P 

PROVIDER: S-EPMC9138872 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature