Establishment and Comparative Analysis of Enzyme-Linked Immunoassay and Time-Resolved Fluoroimmunoassay for the Determination of Trace Quinclorac in Environment.

ABSTRACT: As a common herbicide in farmland, there has been wide concern over quinclorac residue because of its potential risks to the environment and human health. For the detection and monitoring of quinclorac residue in the environment, enzyme-linked immunoassay (ELISA) and time-resolved fluoroimmunoassay (TRFIA) were established. The half-maximal inhibition concentrations (IC₅₀) of ELISA and TRFIA were 0.169 mg/L and 0.087 mg/L with a linear range (IC₂₀-IC₈₀) of 0.020-1.389 mg/L and 0.004-1.861 mg/L, respectively. Compared with ELISA, the limit of detection (LOD, IC₂₀) and IC₅₀ of TRFIA improved approximately 5-fold and 2-fold. The cross-reaction rates for the quinclorac analogs were less than 2%. The average recoveries of quinclorac in rive

SUBMITTER: Liu X 

PROVIDER: S-EPMC9138993 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature