An In-Vitro Study of the Expansion and Transcriptomics of CD⁴⁺ and CD⁸⁺ Naive and Memory T Cells Stimulated by IL-2, IL-7 and IL-15.

ABSTRACT: The growth of T cells ex vivo for the purpose of T cell therapies is a rate-limiting step in the overall process for cancer patients to achieve remission. Growing T cells is a fiscally-, time-, and resource-intensive process. Cytokines have been shown to accelerate the growth of T cells, specifically IL-2, IL-7, and IL-15. Here a design of experiments was conducted to optimize the growth rate of different naïve and memory T cell subsets using combinations of cytokines. Mathematical models were developed to study the impact of IL-2, IL-7, and IL-15 on the growth of T cells. The results show that CD⁴⁺ and CD⁸⁺ naïve T cells grew effectively using moderate IL-2 and IL-7 in combination, and IL-7, respectively. CD⁴⁺ and CD⁸⁺ memory cells favored moder

SUBMITTER: Hopkins B 

PROVIDER: S-EPMC9139303 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature