Orthotopic Transplantation of Human Paediatric High-Grade Glioma in Zebrafish Larvae.

ABSTRACT: Brain tumours are the most common cause of death among children with solid tumours, and high-grade gliomas (HGG) are among the most devastating forms with very poor outcomes. In the search for more effective treatments for paediatric HGG, there is a need for better experimental models. To date, there are no xenograft zebrafish models developed for human paediatric HGG; existing models rely on adult cells. The use of paediatric models is of great importance since it is well known that the genetic and epigenetic mechanisms behind adult and paediatric disease differ greatly. In this study, we present a clinically relevant in vivo model based on paediatric primary glioma stem cell (GSC) cultures, which after orthotopic injection into the zebrafish larvae, can be monitored using confocal imagin

SUBMITTER: Larsson S 

PROVIDER: S-EPMC9139401 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature