Influence of Malignant Pleural Fluid from Lung Adenocarcinoma Patients on Neutrophil Response.

ABSTRACT: Malignant pleural effusion (MPE) is a common severe complication of advanced lung adenocarcinoma (LAC). Neutrophils, an essential component of tumor infiltrates, contribute to tumor progression and their counts in MPE have been associated with worse outcome in LAC. This study aimed to evaluate phenotypical and functional changes of neutrophils induced by MPE to determine the influence of MPE immunomodulatory factors in neutrophil response and to find a possible association between neutrophil functions and clinical outcomes. Pleural fluid samples were collected from 47 LAC and 25 heart failure (HF) patients. We measured neutrophil degranulation products by ELISA, oxidative burst capacity and apoptosis by flow cytometry, and NETosis by fluorescence. The concentration of degranulation product

SUBMITTER: Mulet M 

PROVIDER: S-EPMC9139419 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature