Combining Micropunch Histology and Multidimensional Lipidomic Measurements for In-Depth Tissue Mapping.

ABSTRACT: While decades of technical and analytical advancements have been utilized to discover novel lipid species, increase speciation, and evaluate localized lipid dysregulation at subtissue, cellular, and subcellular levels, many challenges still exist. One limitation is that the acquisition of both in-depth spatial information and comprehensive lipid speciation is extremely difficult, especially when biological material is limited or lipids are at low abundance. In neuroscience, for example, it is often desired to focus on only one brain region or subregion, which can be well under a square millimeter for rodents. Herein, we evaluate a micropunch histology method where cortical brain tissue at 2.0, 1.25, 1.0, 0.75, 0.5, and 0.25 mm diameter sizes and 1 mm depth was collected and analyzed with m

SUBMITTER: Odenkirk MT 

PROVIDER: S-EPMC9139744 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature