Behind the Wall-Compartment-Specific Neovascularisation during Post-Stroke Recovery in Mice.

ABSTRACT: Ischemic stroke is a highly prevalent vascular disease leading to oxygen- and glucose deprivation in the brain. In response, ischemia-induced neovascularization occurs, which is supported by circulating CD34⁺ endothelial progenitor cells. Here, we used the transient middle cerebral artery occlusion (tMCAO) mouse model to characterize the spatio-temporal alterations within the ischemic core from the acute to the chronic phase using multiple-epitope-ligand cartography (MELC) for sequential immunohistochemistry. We found that around 14 days post-stroke, significant angiogenesis occurs in the ischemic core, as determined by the presence of CD31⁺/CD34⁺ double-positive endothelial cells. This neovascularization was accompanied by the recruitment of CD4⁺

SUBMITTER: Kolbinger A 

PROVIDER: S-EPMC9139871 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature