Activation and Migration of Human Skeletal Muscle Stem Cells In Vitro Differently Rely on Calcium Signals.

ABSTRACT: Muscle regeneration is essential for proper muscle homeostasis and relies primarily on muscle stem cells (MuSC). MuSC are maintained quiescent in their niche and can be activated following muscle injury. Using an in vitro model of primary human quiescent MuSC (called reserve cells, RC), we analyzed their Ca²⁺ response following their activation by fetal calf serum and assessed the role of Ca²⁺ in the processes of RC activation and migration. The results showed that RC displayed a high response heterogeneity in a cell-dependent manner following serum stimulation. Most of these responses relied on inositol 1,4,5-trisphosphate (IP₃)-dependent Ca²⁺ release associated with Ca²⁺ influx, partly due to store-operated calcium entry. Our study f

SUBMITTER: Tollance A 

PROVIDER: S-EPMC9140175 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature