Immunomodulatory Role of BLG-Derived Peptides Based on Simulated Gastrointestinal Digestion and DC-T Cell from Mice Allergic to Cow's Milk.

ABSTRACT: Peptides, but not whole protein, elicit an allergic reaction since food allergens should be consumed by digestion. In this study, we explored the remaining peptides after simulated digestion of cow's milk in order to search for β-lactoglobulin (BLG)-derived peptides that could play an immunomodulatory role. As a major allergen in milk, BLG-derived peptides, 109 in total, were identified both from simulated infant and adult digestion in vitro. These peptides were mainly located in four regions, and they were synthesized as five peptides, namely, BLG_1-14, BLG_24-35, BLG_40-60, BLG_82-101, and BLG_123-139. Then, the effect of peptides on the Caco-2 cell's transport absorption, the co-stimulatory molecules of DC, and the T-cell phenotype was e

SUBMITTER: Ma X 

PROVIDER: S-EPMC9140701 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature