Identification of the CKM Gene as a Potential Muscle-Specific Safe Harbor Locus in Pig Genome.

ABSTRACT: Genetically modified pigs have shown considerable application potential in the fields of life science research and livestock breeding. Nevertheless, a barrier impedes the production of genetically modified pigs. There are too few safe harbor loci for the insertion of foreign genes into the pig genome. Only a few loci (pRosa26, pH11 and Pifs501) have been successfully identified to achieve the ectopic expression of foreign genes and produce gene-edited pigs. Here, we use CRISPR/Cas9-mediated homologous directed repair (HDR) to accurately knock the exogenous gene-of-interest fragments into an endogenous CKM gene in the porcine satellite cells. After porcine satellite cells are induced to differentiate, the CKM gene promoter simultaneously initiates the expr

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC9140944 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature